Hotspot

rpl32-ndhF

SSC · 131385-132406 · 1022 bp

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Pi 0.0137

MarkerSeek score 57.30

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1022
variable_sites: 40
pi: 0.0137
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	TGAAAAAGCTTTCAACGCCG	GGGAAGTGGCACAAAAGGTA	45.0	50.0	58.2	58.0	1171-1309 bp	1.0	82.0
rpl32-ndhF_p2	CCGCCCGATATCCTTTCTTT	GGGAAGTGGCACAAAAGGTA	50.0	50.0	57.7	58.0	1154-1292 bp	1.0	81.3
rpl32-ndhF_p3	TGAAAAAGCTTTCAACGCCG	ACAAACGCTTTTTGACAGGC	45.0	45.0	58.2	58.1	1659-1797 bp	1.0	80.7
rpl32-ndhF_p4	CCGCCCGATATCCTTTCTTT	ACAAACGCTTTTTGACAGGC	50.0	45.0	57.7	58.1	1642-1780 bp	1.0	80.0
rpl32-ndhF_p5	TGAAAAAGCTTTCAACGCCG	TGAAGAGGAAATTGCGCAGA	45.0	45.0	58.2	57.8	1860-1998 bp	1.0	79.6