Hotspot

rps19-rpl2

IRb · 88943-89102 · 160 bp

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Pi 0.0665

MarkerSeek score 73.30

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 160
variable_sites: 28
pi: 0.0665
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TGGTAGATGCTCTGGACCAA	TTGATTCTTCGTCGCCGTAG	50.0	50.0	58.0	58.1	237-305 bp	1.0	90.8
rps19-rpl2_p2	TGGTAGATGCTCTGGACCAA	AAAAGTTTGGGTAGAGCCGG	50.0	50.0	58.0	58.1	435-503 bp	1.0	88.0
rps19-rpl2_p3	TGGTAGATGCTCTGGACCAA	ATTACCTTCTGGGGAGGTCC	50.0	55.0	58.0	58.1	517-585 bp	1.0	87.2
rps19-rpl2_p4	TGGTAGATGCTCTGGACCAA	TGGGGAGGTCCGTTTGATAT	50.0	50.0	58.0	58.1	508-576 bp	1.0	87.2
rps19-rpl2_p5	TGCTGCTGCAGAATAAACCA	AAAAGTTTGGGTAGAGCCGG	45.0	50.0	58.1	58.1	864-958 bp	1.0	85.6