Hotspot

psbT-pbf1

LSC · 78589-78662 · 74 bp

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Pi 0.0528

MarkerSeek score 72.10

Primer available yes

Label psbT-pbf1

Feature Metadata

label_name: psbT-pbf1
feature_type: igs
region: LSC
length_bp: 74
variable_sites: 9
pi: 0.0528
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-pbf1_p1	TCGAGAACCGCCTAAAGTTC	GAGATCCGTTCGAGGAACAC	50.0	55.0	57.6	58.1	137 bp	1.0	81.7
psbT-pbf1_p2	TCGAGAACCGCCTAAAGTTC	AGAGATCCGTTCGAGGAACA	50.0	50.0	57.6	58.2	138 bp	1.0	81.0
psbT-pbf1_p3	GAGAACCGCCTAAAGTTCCA	GAGATCCGTTCGAGGAACAC	50.0	55.0	57.5	58.1	135 bp	1.0	80.9
psbT-pbf1_p4	GAGAACCGCCTAAAGTTCCA	AGAGATCCGTTCGAGGAACA	50.0	50.0	57.5	58.2	136 bp	1.0	80.2
psbT-pbf1_p5	CGAGAACCGCCTAAAGTTCC	GAGATCCGTTCGAGGAACAC	55.0	55.0	58.6	58.1	136 bp	1.0	79.4