Hotspot

psaJ-rpl33

LSC · 71238-71785 · 548 bp

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Pi 0.016

MarkerSeek score 62.00

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 548
variable_sites: 23
pi: 0.016
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TCGTTTCTTTCCAGATGCGT	CATCTTTACTCTTGGCCAT	45.0	42.1	57.8	52.2	573-611 bp	1.0	47.1
psaJ-rpl33_p2	CGTTTCTTTCCAGATGCGTT	CATCTTTACTCTTGGCCAT	45.0	42.1	57.0	52.2	572-610 bp	1.0	47.1
psaJ-rpl33_p3	CCAGATGCGTTAACATTCCC	CATCTTTACTCTTGGCCAT	50.0	42.1	56.9	52.2	563-601 bp	1.0	47.1
psaJ-rpl33_p4	ATCGTTTCTTTCCAGATGCGT	CATCTTTACTCTTGGCCAT	42.9	42.1	58.3	52.2	574-612 bp	1.0	47.1
psaJ-rpl33_p5	CCAGATGCGTTAACATTCCCT	CATCTTTACTCTTGGCCAT	47.6	42.1	58.4	52.2	563-601 bp	1.0	47.1