Hotspot

petG-trnW-CCA

LSC · 70251-70409 · 159 bp

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Pi 0.0202

MarkerSeek score 64.50

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 159
variable_sites: 8
pi: 0.0202
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	ACAGACGTGGTGATCAGTTG	AGAACGTGGGTCTCCAAAAC	50.0	50.0	57.8	58.0	235-266 bp	1.0	83.7
petG-trnW-CCA_p2	ACAGACGTGGTGATCAGTTG	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.8	58.0	257-288 bp	1.0	83.4
petG-trnW-CCA_p3	ACAGACGTGGTGATCAGTTG	CGCTCTTAGTTCAGTTCGGT	50.0	50.0	57.8	57.7	255-286 bp	1.0	80.8
petG-trnW-CCA_p4	GTGGTGATCAGTTGGACCTT	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.4	58.0	251-282 bp	1.0	80.2
petG-trnW-CCA_p5	CAGACGTGGTGATCAGTTGG	AGCGCTCTTAGTTCAGTTCG	55.0	50.0	58.6	58.0	256-287 bp	1.0	80.1