Hotspot

pafII-cemA

LSC · 64269-65322 · 1054 bp

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Pi 0.0108

MarkerSeek score 65.90

Primer available yes

Label pafII-cemA

Feature Metadata

label_name: pafII-cemA
feature_type: igs
region: LSC
length_bp: 1054
variable_sites: 35
pi: 0.0108
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pafII-cemA_p1	GCGTGTACCGATTGAAGTCT	GAGATCCACCAGGGCAAAAA	50.0	50.0	58.0	58.1	1065-1161 bp	1.0	85.8
pafII-cemA_p2	TGACTCCGCGAGAAATTGAG	GAGATCCACCAGGGCAAAAA	50.0	50.0	58.0	58.1	1114-1210 bp	1.0	85.7
pafII-cemA_p3	TGAGAATTTGACTCCGCGAG	GAGATCCACCAGGGCAAAAA	50.0	50.0	58.0	58.1	1122-1218 bp	1.0	85.7
pafII-cemA_p4	CGTTCCCTTGACTCGTACTG	GAGATCCACCAGGGCAAAAA	55.0	50.0	58.0	58.1	1143-1239 bp	1.0	85.5
pafII-cemA_p5	TTGCGTGTACCGATTGAAGT	GAGATCCACCAGGGCAAAAA	45.0	50.0	57.8	58.1	1067-1163 bp	1.0	84.6