Hotspot

trnR-UCU-atpA

LSC · 10505-10684 · 180 bp

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Pi 0.0194

MarkerSeek score 63.70

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 180
variable_sites: 10
pi: 0.0194
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	GAAACCCTTTTGAAAGACGCT	47.6	42.9	57.3	57.3	241-276 bp	1.0	59.8
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	AAACCCTTTTGAAAGACGCT	47.6	40.0	57.3	56.1	240-275 bp	1.0	58.0
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	ACGCTATTCAGGAACAAATGGA	47.6	40.9	57.3	58.0	225-260 bp	1.0	57.3
trnR-UCU-atpA_p4	TGGATAGGACAGAGGTCTTCT	GAAACCCTTTTGAAAGACGCT	47.6	42.9	56.9	57.3	276-311 bp	1.0	56.6
trnR-UCU-atpA_p5	GGTTCAAATCCTATTGGACGC	GAAACCCTTTTGAAAGACGC	47.6	45.0	57.3	55.8	241-276 bp	1.0	55.7