Hotspot

trnK-UUU-rps16

LSC · 4686-5577 · 892 bp

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Pi 0.0176

MarkerSeek score 60.70

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 892
variable_sites: 33
pi: 0.0176
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCTTTCAGGATCAGTCGTGG	AAAAGGAGCTCAACCCACAG	55.0	50.0	58.0	58.0	925-1115 bp	0.857143	80.6
trnK-UUU-rps16_p2	ATACCATACCTCGGGGACAG	AAAAGGAGCTCAACCCACAG	55.0	50.0	58.0	58.0	1489-1668 bp	0.857143	80.4
trnK-UUU-rps16_p3	GATACCATACCTCGGGGACA	AAAAGGAGCTCAACCCACAG	55.0	50.0	58.0	58.0	1632-1669 bp	0.714286	72.3
trnK-UUU-rps16_p4	GATACCATACCTCGGGGACA	CCCAATGAGCCGTTTATCGA	55.0	50.0	58.0	58.1	1771-1808 bp	0.714286	71.7
trnK-UUU-rps16_p5	GATACCATACCTCGGGGACA	TCCCAATGAGCCGTTTATCG	55.0	50.0	58.0	58.1	1772-1809 bp	0.714286	71.7