Hotspot

ycf4

LSC · 60961-61593 · 633 bp

Pi 0.0332

MarkerSeek score 68.00

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: LSC
length_bp: 633
variable_sites: 20
pi: 0.0332
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	ATTTCCGGCAATTGCAATGG	ATCCACCGTCAAGTCGAATG	45.0	50.0	58.0	58.0	1174-1192 bp	1.0	85.4
ycf4_p2	ATTTCCGGCAATTGCAATGG	GAATCCACCGTCAAGTCGAA	45.0	50.0	58.0	57.9	1176-1194 bp	1.0	84.9
ycf4_p3	ATTTCCGGCAATTGCAATGG	GGGAGTTAGTCCTTGCCTTG	45.0	55.0	58.0	57.9	1226-1244 bp	1.0	84.4
ycf4_p4	ATTTCCGGCAATTGCAATGG	CGTGTTTCCGGGTATTCCTT	45.0	50.0	58.0	57.9	1616-1629 bp	1.0	83.6
ycf4_p5	GCAATTGCAATGGCTTCCTT	ATCCACCGTCAAGTCGAATG	45.0	50.0	57.9	58.0	1185 bp	0.5	59.1