Hotspot

ycf1

SSC · 125785-131190 · 5406 bp

Back to Abrus

Pi 0.0457
MarkerSeek score 69.30
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5406
variable_sites
245
pi
0.0457
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 GTCCGTTCAGCAAAGGATCA ATGGAAGAAGGGACCGAGAA 50.0 50.0 58.2 58.0 1201-1210 bp 1.0 82.7
ycf1_p2 GTCCGTTCAGCAAAGGATCA CTCTTCCTTCTTCGAGCTCG 50.0 55.0 58.2 57.8 1225-1234 bp 1.0 81.5
ycf1_p3 GTCCGTTCAGCAAAGGATCA ACTGCCATAGCTCTACCGTA 50.0 50.0 58.2 57.6 1078-1087 bp 1.0 80.5
ycf1_p4 GTCCGTTCAGCAAAGGATCA GGGACAGCTCATGATGTTCA 50.0 50.0 58.2 57.6 1151-1160 bp 1.0 80.0
ycf1_p5 GTCCGTTCAGCAAAGGATCA TGGGTTTATTACGGGACAGC 50.0 50.0 58.2 57.6 1163-1172 bp 1.0 79.9