Hotspot

ndhF

SSC · 112344-114587 · 2244 bp

Pi 0.0263

MarkerSeek score 57.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2244
variable_sites: 59
pi: 0.0263
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ATGGAAGAAGGGACCGAGAA	AACCGACCCTTTTCTTTACA	50.0	40.0	58.0	54.8	2661-2683 bp	1.0	57.0
ndhF_p2	ATGGAAGAAGGGACCGAGAA	ACCGACCCTTTTCTTTACAA	50.0	40.0	58.0	54.8	2660-2682 bp	1.0	57.0
ndhF_p3	ATCGTTGTGGTCGGACTCTA	ACCGACCCTTTTCTTTACAA	50.0	40.0	58.2	54.8	2744-2766 bp	1.0	55.9
ndhF_p4	TCGTTGTGGTCGGACTCTAT	AACCGACCCTTTTCTTTACA	50.0	40.0	58.2	54.8	2744-2766 bp	1.0	55.9
ndhF_p5	TCGTTGTGGTCGGACTCTAT	ACCGACCCTTTTCTTTACAA	50.0	40.0	58.2	54.8	2743-2765 bp	1.0	55.9