Hotspot

ndhD

SSC · 117341-118837 · 1497 bp

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Pi 0.0194
MarkerSeek score 56.40
Primer available yes
Label ndhD

Feature Metadata

label_name
ndhD
feature_type
gene
region
SSC
length_bp
1497
variable_sites
29
pi
0.0194
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD_p1 TCAGGAGCAGTATGGGCTAA AAATTGCTTCTGCCCCAAGA 50.0 45.0 57.8 58.0 2313 bp 1.0 80.8
ndhD_p2 TCAGGAGCAGTATGGGCTAA AATTGCTTCTGCCCCAAGAA 50.0 45.0 57.8 58.0 2312 bp 1.0 80.8
ndhD_p3 TTCAGGAGCAGTATGGGCTA AAATTGCTTCTGCCCCAAGA 50.0 45.0 57.8 58.0 2314 bp 1.0 80.8
ndhD_p4 TTCAGGAGCAGTATGGGCTA AATTGCTTCTGCCCCAAGAA 50.0 45.0 57.8 58.0 2313 bp 1.0 80.8
ndhD_p5 TCAGGAGCAGTATGGGCTAA TGAGACAACTCGAAGCATGG 50.0 50.0 57.8 57.9 2213 bp 1.0 80.3