Hotspot

rpl32-trnL-UAG

SSC · 115473-115891 · 419 bp

Back to Abrus

Pi 0.063
MarkerSeek score 80.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
419
variable_sites
24
pi
0.063
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGAGGGGATATTGGACAACA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 55.5 57.9 553-571 bp 1.0 66.4
rpl32-trnL-UAG_p2 AGAGGGGATATTGGACAACA AGCAGCGTGTCTACCAATTT 45.0 45.0 55.5 57.8 546-564 bp 1.0 65.9
rpl32-trnL-UAG_p3 AGAGGGGATATTGGACAACA CCTAAGAGCAGCGTGTCTAC 45.0 55.0 55.5 57.8 552-570 bp 1.0 65.8
rpl32-trnL-UAG_p4 AGAGGGGATATTGGACAACA CTAAGAGCAGCGTGTCTACC 45.0 55.0 55.5 57.8 551-569 bp 1.0 65.8
rpl32-trnL-UAG_p5 AGAGGGGATATTGGACAACA GAGCAGCGTGTCTACCAATT 45.0 50.0 55.5 58.3 547-565 bp 1.0 65.2