Hotspot

ndhF-rpl32

SSC · 114588-115295 · 708 bp

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Pi 0.0496
MarkerSeek score 75.60
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
708
variable_sites
34
pi
0.0496
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GCCCATATACGGCGAAGATT TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.9 57.9 1448-1465 bp 1.0 85.3
ndhF-rpl32_p2 TTTGCTGCAATAGGACGTGT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 1844-1861 bp 1.0 83.9
ndhF-rpl32_p3 TGTTGCCGTCGGAAAAAGTA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.0 57.9 1442 bp 0.5 58.6
ndhF-rpl32_p4 TGTTGCCGTCGGAAAAAGTA AGCAGCGTGTCTACCAATTT 45.0 45.0 58.0 57.8 1435 bp 0.5 58.1
ndhF-rpl32_p5 TGTTGCCGTCGGAAAAAGTA CTAAGAGCAGCGTGTCTACC 45.0 55.0 58.0 57.8 1440 bp 0.5 58.0