Hotspot

trnP-UGG-psaJ

LSC · 68177-68705 · 529 bp

Back to Abrus

Pi 0.051
MarkerSeek score 80.80
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
529
variable_sites
26
pi
0.051
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 ACCCGTGACATTTTGTACCC TGGTGCCACGGAAAGATATG 50.0 50.0 58.1 58.0 620-631 bp 1.0 84.7
trnP-UGG-psaJ_p2 CCCGTGACATTTTGTACCCA TGGTGCCACGGAAAGATATG 50.0 50.0 58.1 58.0 619-630 bp 1.0 84.7
trnP-UGG-psaJ_p3 ACCCGTGACATTTTGTACCC GGTGCCACGGAAAGATATGT 50.0 50.0 58.1 58.0 619-630 bp 1.0 84.7
trnP-UGG-psaJ_p4 CCCGTGACATTTTGTACCCA GGTGCCACGGAAAGATATGT 50.0 50.0 58.1 58.0 618-629 bp 1.0 84.7
trnP-UGG-psaJ_p5 ACAGGATTTGAACCCGTGAC TGGTGCCACGGAAAGATATG 50.0 50.0 58.1 58.0 631-642 bp 1.0 84.5