Hotspot

trnQ-UUG-rps16

LSC · 56396-56860 · 465 bp

Back to Abrus

Pi 0.0486
MarkerSeek score 76.90
Primer available yes
Label trnQ-UUG-rps16

Feature Metadata

label_name
trnQ-UUG-rps16
feature_type
igs
region
LSC
length_bp
465
variable_sites
21
pi
0.0486
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-rps16_p1 GAGGTTCGAATCCTTCCGTC GTCTTTCAAGTCGCACGTTG 55.0 50.0 58.1 58.0 539-545 bp 1.0 86.4
trnQ-UUG-rps16_p2 GAGGTTCGAATCCTTCCGTC ATGTCTTTCAAGTCGCACGT 55.0 45.0 58.1 58.1 541-547 bp 1.0 85.4
trnQ-UUG-rps16_p3 GAGGTTCGAATCCTTCCGTC GCACGTTGCTTTTTACCACA 55.0 45.0 58.1 57.8 527-533 bp 1.0 85.0
trnQ-UUG-rps16_p4 GAGGTTCGAATCCTTCCGTC CATGTCTTTCAAGTCGCACG 55.0 50.0 58.1 57.8 542-548 bp 1.0 84.7
trnQ-UUG-rps16_p5 GAGGTTCGAATCCTTCCGTC GGCGGATGTAAGAATCCACA 55.0 50.0 58.1 57.7 573-579 bp 1.0 83.7