Hotspot

atpI-atpH

LSC · 47572-48981 · 1410 bp

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Pi 0.0424
MarkerSeek score 78.30
Primer available yes
Label atpI-atpH

Feature Metadata

label_name
atpI-atpH
feature_type
igs
region
LSC
length_bp
1410
variable_sites
59
pi
0.0424
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI-atpH_p1 GCTGCGGCTTATATAGGTGA ATAACGGAAGCGGCAGAAAT 50.0 45.0 57.3 58.0 1484-1559 bp 1.0 77.8
atpI-atpH_p2 GCTGCGGCTTATATAGGTGA CGCAATCCCTTCTACAGCTT 50.0 50.0 57.3 58.0 1572-1647 bp 1.0 77.6
atpI-atpH_p3 ACAAGCGGTATTCAAGCTCT ATAACGGAAGCGGCAGAAAT 45.0 45.0 57.2 58.0 1520-1595 bp 1.0 77.5
atpI-atpH_p4 ACAAGCGGTATTCAAGCTCT CGCAATCCCTTCTACAGCTT 45.0 50.0 57.2 58.0 1608-1683 bp 1.0 77.3
atpI-atpH_p5 GCTGCGGCTTATATAGGTGA AATAACGGAAGCGGCAGAAA 50.0 45.0 57.3 58.2 1485-1560 bp 1.0 76.6