Hotspot

rpoB-rpoC1

LSC · 38395-38420 · 26 bp

Back to Abrus

Pi 0.0769
MarkerSeek score 79.50
Primer available yes
Label rpoB-rpoC1

Feature Metadata

label_name
rpoB-rpoC1
feature_type
igs
region
LSC
length_bp
26
variable_sites
2
pi
0.0769
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-rpoC1_p1 TTGCTCGTTCGAGAACTACG CCTCCCCAACTATCTCTCCA 50.0 55.0 58.0 57.5 228 bp 1.0 76.5
rpoB-rpoC1_p2 TTGCTCGTTCGAGAACTACG ACCTCCCCAACTATCTCTCC 50.0 55.0 58.0 57.5 229 bp 1.0 76.5
rpoB-rpoC1_p3 TTCGATTGCTCGTTCGAGAA CCTCCCCAACTATCTCTCCA 45.0 55.0 57.7 57.5 233 bp 1.0 74.1
rpoB-rpoC1_p4 TTTCGATTGCTCGTTCGAGA CCTCCCCAACTATCTCTCCA 45.0 55.0 57.7 57.5 234 bp 1.0 74.1
rpoB-rpoC1_p5 TTCGATTGCTCGTTCGAGAA ACCTCCCCAACTATCTCTCC 45.0 55.0 57.7 57.5 234 bp 1.0 74.1