Hotspot

trnC-GCA-rpoB

LSC · 34000-35181 · 1182 bp

Back to Abrus

Pi 0.0455
MarkerSeek score 73.00
Primer available yes
Label trnC-GCA-rpoB

Feature Metadata

label_name
trnC-GCA-rpoB
feature_type
igs
region
LSC
length_bp
1182
variable_sites
53
pi
0.0455
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-rpoB_p1 CCGGATTTGAACTGGGGAAA CCTCATTTCCAACCTCAAGC 50.0 50.0 58.1 56.7 1265-1266 bp 1.0 73.3
trnC-GCA-rpoB_p2 CCGGATTTGAACTGGGGAAA TCCCTCATTTCCAACCTCAAG 50.0 47.6 58.1 57.6 1267-1268 bp 1.0 72.2
trnC-GCA-rpoB_p3 AATCTCTTGTCGATCAGGCG CCTCATTTCCAACCTCAAGC 50.0 50.0 57.8 56.7 1289-1290 bp 1.0 72.1
trnC-GCA-rpoB_p4 CCGGATTTGAACTGGGGAAA CCTCATTTCCAACCTCAAGCA 50.0 47.6 58.1 58.5 1265-1266 bp 1.0 71.9
trnC-GCA-rpoB_p5 AATCTCTTGTCGATCAGGCG TCCCTCATTTCCAACCTCAAG 50.0 47.6 57.8 57.6 1291-1292 bp 1.0 71.1