Hotspot

trnH-GUG-psbA

LSC · 133-393 · 261 bp

Back to Abrus

Pi 0.104
MarkerSeek score 76.70
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
261
variable_sites
25
pi
0.104
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 ATCCACTTGGTTACATCCGC GTTGACAGTCAAGGTCGTGT 50.0 50.0 58.0 58.1 405-425 bp 1.0 88.2
trnH-GUG-psbA_p2 ATCCACTTGGTTACATCCGC GGTGTAGCTGGTGTATTCGG 50.0 55.0 58.0 58.1 723-743 bp 1.0 84.4
trnH-GUG-psbA_p3 ATCCACTTGGTTACATCCGC CGGATGGTATGCCTCTAGGA 50.0 55.0 58.0 58.1 818-838 bp 1.0 83.7
trnH-GUG-psbA_p4 ATCCACTTGGTTACATCCGC TCGGATGGTATGCCTCTAGG 50.0 55.0 58.0 58.1 819-839 bp 1.0 83.7
trnH-GUG-psbA_p5 ATCCACTTGGTTACATCCGC GGTCAAGGAAGCTTTTCGGA 50.0 50.0 58.0 58.1 834-854 bp 1.0 83.5