Hotspot

ycf1

Genome · 96061-101958 · 5898 bp

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Pi 0.00149
MarkerSeek score 55.10
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
Genome
length_bp
5898
variable_sites
56
pi
0.00149
unique_haplotype_count
16
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 GAAGAACGGAAGCAAACAACA AGCTTGTACCGTTTTGTATTGA 42.9 36.4 57.6 56.3 330-354 bp 1.0 47.2
ycf1_p2 ACGGAAGCAAACAACAAATGT AGCTTGTACCGTTTTGTATTGA 38.1 36.4 57.5 56.3 325-349 bp 1.0 47.2
ycf1_p3 TGGAACAAGGAATAGGTGCT AGCTTGTACCGTTTTGTATTGA 45.0 36.4 56.4 56.3 302-326 bp 1.0 47.2
ycf1_p4 AAGAACGGAAGCAAACAACA AGCTTGTACCGTTTTGTATTGA 40.0 36.4 56.4 56.3 329-353 bp 1.0 47.2
ycf1_p5 AGAACGGAAGCAAACAACAA AGCTTGTACCGTTTTGTATTGA 40.0 36.4 56.4 56.3 328-352 bp 1.0 47.2