Hotspot

ycf2-trnH-GUG

Genome · 120306-120629 · 324 bp

Back to Abies

Pi 0.00751
MarkerSeek score 50.10
Primer available yes
Label ycf2-trnH-GUG

Feature Metadata

label_name
ycf2-trnH-GUG
feature_type
igs
region
Genome
length_bp
324
variable_sites
13
pi
0.00751
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf2-trnH-GUG_p1 GGATTCGGTCCATTGATTGC CCAAGGCAGTGGATTGTGAA 50.0 50.0 57.2 58.4 457-577 bp 1.0 73.7
ycf2-trnH-GUG_p2 GGATTCGGTCCATTGATTGC TTGGTTTGTTTTTACGGGGC 50.0 45.0 57.2 57.4 494-614 bp 1.0 71.8
ycf2-trnH-GUG_p3 GGATTCGGTCCATTGATTGC GGGTTCAATTCCCGTCGTT 50.0 52.6 57.2 58.1 425-545 bp 1.0 68.5
ycf2-trnH-GUG_p4 TCTCACCAAATTGGATTCGGT CCAAGGCAGTGGATTGTGAA 42.9 50.0 57.6 58.4 469-589 bp 1.0 68.4
ycf2-trnH-GUG_p5 GGATTCGGTCCATTGATTGC ACCAAGGCAGTGGATTGTG 50.0 52.6 57.2 58.0 458-578 bp 1.0 68.4