Hotspot

rps7-trnL-CAA

Genome · 111791-113253 · 1463 bp

Back to Abies

Pi 0.0017
MarkerSeek score 50.00
Primer available yes
Label rps7-trnL-CAA

Feature Metadata

label_name
rps7-trnL-CAA
feature_type
igs
region
Genome
length_bp
1463
variable_sites
15
pi
0.0017
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps7-trnL-CAA_p1 GCAGAGGCCAATAGAGCTTT GTCTCGCGTGTCTACCATTT 50.0 50.0 58.0 58.0 1530-1573 bp 1.0 80.5
rps7-trnL-CAA_p2 GCAGAGGCCAATAGAGCTTT CTCGCGTGTCTACCATTTCA 50.0 50.0 58.0 58.0 1528-1571 bp 1.0 80.5
rps7-trnL-CAA_p3 GCAGAGGCCAATAGAGCTTT GCGTGTCTACCATTTCACCA 50.0 50.0 58.0 58.2 1525-1568 bp 1.0 79.0
rps7-trnL-CAA_p4 GCAGAGGCCAATAGAGCTTT TCTCGCGTGTCTACCATTTC 50.0 50.0 58.0 57.7 1529-1572 bp 1.0 78.3
rps7-trnL-CAA_p5 GCAGAGGCCAATAGAGCTTT TCGCGTGTCTACCATTTCAC 50.0 50.0 58.0 58.3 1527-1570 bp 1.0 78.2