Hotspot

rpl32-trnV-GAC

Genome · 108016-108814 · 799 bp

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Pi 0.00634
MarkerSeek score 62.80
Primer available yes
Label rpl32-trnV-GAC

Feature Metadata

label_name
rpl32-trnV-GAC
feature_type
igs
region
Genome
length_bp
799
variable_sites
27
pi
0.00634
unique_haplotype_count
13
primer_available
yes
alignment_reliability
0.98
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnV-GAC_p1 ATTGCGGACCGATATGCTTT TTGACGTGGTGGAAGTCATC 45.0 50.0 58.0 57.8 910-927 bp 1.0 83.7
rpl32-trnV-GAC_p2 ATTGCGGACCGATATGCTTT CGTGGTGGAAGTCATCAGTT 45.0 50.0 58.0 57.8 906-923 bp 1.0 83.7
rpl32-trnV-GAC_p3 ATTGCGGACCGATATGCTTT CTTGACGTGGTGGAAGTCAT 45.0 50.0 58.0 57.8 911-928 bp 1.0 83.7
rpl32-trnV-GAC_p4 AGCTTTTATTGCGGACCGAT TTGACGTGGTGGAAGTCATC 45.0 50.0 58.0 57.8 917-934 bp 1.0 83.6
rpl32-trnV-GAC_p5 AGCTTTTATTGCGGACCGAT CGTGGTGGAAGTCATCAGTT 45.0 50.0 58.0 57.8 913-930 bp 1.0 83.6