Hotspot

psbD-trnT-GGU

Genome · 82901-84214 · 1314 bp

Back to Abies

Pi 0.0015
MarkerSeek score 53.60
Primer available yes
Label psbD-trnT-GGU

Feature Metadata

label_name
psbD-trnT-GGU
feature_type
igs
region
Genome
length_bp
1314
variable_sites
15
pi
0.0015
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbD-trnT-GGU_p1 ATAGACCAGACCAACCCACA GGCGTAAGTCATCGGTTCAA 50.0 50.0 58.0 58.3 1455-1461 bp 1.0 78.6
psbD-trnT-GGU_p2 ATAGACCAGACCAACCCACA ATGGGGGCCTTTTCAATTCA 50.0 45.0 58.0 57.7 1498-1504 bp 1.0 78.2
psbD-trnT-GGU_p3 ATAGACCAGACCAACCCACA TGGGGGCCTTTTCAATTCAG 50.0 50.0 58.0 58.4 1497-1503 bp 1.0 78.1
psbD-trnT-GGU_p4 ATAGACCAGACCAACCCACA AATGATGGGGGCCTTTTCAA 50.0 45.0 58.0 57.7 1505 bp 0.058824 39.3
psbD-trnT-GGU_p5 ATAGACCAGACCAACCCACA TGATGGGGGCCTTTTCAATT 50.0 45.0 58.0 57.7 1503 bp 0.058824 39.3