Hotspot

rpl14-rpl16

Genome · 62093-62210 · 118 bp

Back to Abies

Pi 0.00991
MarkerSeek score 52.20
Primer available yes
Label rpl14-rpl16

Feature Metadata

label_name
rpl14-rpl16
feature_type
igs
region
Genome
length_bp
118
variable_sites
3
pi
0.00991
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.98
markerseek_score
52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl14-rpl16_p1 ATTTTTCGGGCTCCACTGTT GTCATCAGACCAGGTCGAAT 45.0 50.0 58.0 57.0 275-294 bp 1.0 77.0
rpl14-rpl16_p2 ATTTTTCGGGCTCCACTGTT GAAACCGTCGCTAGAGCAG 45.0 57.9 58.0 58.3 230-249 bp 1.0 74.2
rpl14-rpl16_p3 TTTCGGGCTCCACTGTTATC GTCATCAGACCAGGTCGAAT 50.0 50.0 57.6 57.0 272-291 bp 1.0 73.9
rpl14-rpl16_p4 ATTTTTCGGGCTCCACTGTT ACTTTATGAAATGGGCGGAGT 45.0 42.9 58.0 57.6 255-274 bp 1.0 73.9
rpl14-rpl16_p5 TTTTTCGGGCTCCACTGTTA GTCATCAGACCAGGTCGAAT 45.0 50.0 57.4 57.0 274-293 bp 1.0 71.9