Hotspot

atpH-atpF

Genome · 47654-47996 · 343 bp

Back to Abies

Pi 0.0101
MarkerSeek score 36.70
Primer available yes
Label atpH-atpF

Feature Metadata

label_name
atpH-atpF
feature_type
igs
region
Genome
length_bp
343
variable_sites
6
pi
0.0101
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.73
markerseek_score
36.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpF_p1 TGTGGCCCTAGCACTTCTAT CCAGCAGAAGGCCAATAACT 50.0 50.0 58.1 57.9 338-436 bp 1.0 83.3
atpH-atpF_p2 ATGGACTAGTTGTGGCCCTA CCAGCAGAAGGCCAATAACT 50.0 50.0 57.7 57.9 348-446 bp 1.0 82.1
atpH-atpF_p3 TATGGACTAGTTGTGGCCCT CCAGCAGAAGGCCAATAACT 50.0 50.0 57.7 57.9 349-447 bp 1.0 82.1
atpH-atpF_p4 AGCGGAAGGTAAAATACGGG CCAGCAGAAGGCCAATAACT 50.0 50.0 57.7 57.9 413-511 bp 1.0 81.5
atpH-atpF_p5 TTGTGGCCCTAGCACTTCTA CCAGCAGAAGGCCAATAACT 50.0 50.0 58.3 57.9 339-437 bp 1.0 81.5