Hotspot

trnV-UAC-trnE-UUC

Genome · 27293-31582 · 4290 bp

Back to Abies

Pi 0.00587
MarkerSeek score 58.00
Primer available yes
Label trnV-UAC-trnE-UUC

Feature Metadata

label_name
trnV-UAC-trnE-UUC
feature_type
igs
region
Genome
length_bp
4290
variable_sites
109
pi
0.00587
unique_haplotype_count
17
primer_available
yes
alignment_reliability
0.88
markerseek_score
58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-UAC-trnE-UUC_p1 ACGGGAAAAGCGGGTAATTT TAAAACGCCACATGTCCGAT 45.0 45.0 58.1 57.9 172-214 bp 0.529412 68.9
trnV-UAC-trnE-UUC_p2 ACGGGAAAAGCGGGTAATTT GCAAAGGGATTGAACGAACG 45.0 50.0 58.1 58.0 242-284 bp 0.529412 67.1
trnV-UAC-trnE-UUC_p3 ACGGGAAAAGCGGGTAATTT GGGATTGAACGAACGGAAGA 45.0 50.0 58.1 57.9 237-279 bp 0.529412 66.9
trnV-UAC-trnE-UUC_p4 ACGGGAAAAGCGGGTAATTT AGGGATTGAACGAACGGAAG 45.0 50.0 58.1 57.9 238-280 bp 0.529412 66.8
trnV-UAC-trnE-UUC_p5 AAGCGGGTAATTTGTCACGA GCAAAGGGATTGAACGAACG 45.0 50.0 57.8 58.0 235-277 bp 0.529412 66.6