Hotspot

cemA-ycf4

Genome · 18489-18932 · 444 bp

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Pi 0.00786
MarkerSeek score 60.50
Primer available yes
Label cemA-ycf4

Feature Metadata

label_name
cemA-ycf4
feature_type
igs
region
Genome
length_bp
444
variable_sites
14
pi
0.00786
unique_haplotype_count
14
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
cemA-ycf4_p1 CACTTCCAATTCGCGAATCG AACAGAAAGCTGCCGAATCA 50.0 45.0 57.9 58.1 594-616 bp 1.0 81.8
cemA-ycf4_p2 CACTTCCAATTCGCGAATCG TATCGAACAGAAAGCTGCCG 50.0 50.0 57.9 58.4 599-621 bp 1.0 79.7
cemA-ycf4_p3 CTCGTCAGTTCTGTCCGAAA AACAGAAAGCTGCCGAATCA 50.0 45.0 57.6 58.1 557-579 bp 1.0 79.3
cemA-ycf4_p4 CACTTCCAATTCGCGAATCG AAGGGCCTACAAAACATCCC 50.0 50.0 57.9 57.8 660 bp 0.058824 39.9
cemA-ycf4_p5 CACTTCCAATTCGCGAATCG GGGCCTACAAAACATCCCTT 50.0 50.0 57.9 57.8 658 bp 0.058824 39.9