Hotspot

petL-psbE

Genome · 13438-14702 · 1265 bp

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Pi 0.00232
MarkerSeek score 53.40
Primer available yes
Label petL-psbE

Feature Metadata

label_name
petL-psbE
feature_type
igs
region
Genome
length_bp
1265
variable_sites
17
pi
0.00232
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petL-psbE_p1 AGTAGACGTGGAAGGACTGA TTCTCCCGTATTCCCAGACA 50.0 50.0 57.4 57.8 1327-1336 bp 1.0 75.0
petL-psbE_p2 AGTAGACGTGGAAGGACTGA AATGTCAGCAAAGGAGCGTT 50.0 45.0 57.4 58.4 1345-1354 bp 1.0 73.8
petL-psbE_p3 GACGTGGAAGGACTGAATGA TTCTCCCGTATTCCCAGACA 50.0 50.0 57.3 57.8 1323-1332 bp 1.0 73.7
petL-psbE_p4 AGACGTGGAAGGACTGAATG TTCTCCCGTATTCCCAGACA 50.0 50.0 57.2 57.8 1324-1333 bp 1.0 73.6
petL-psbE_p5 AGTAGACGTGGAAGGACTGA TCTCCCGTATTCCCAGACAT 50.0 50.0 57.4 57.5 1326-1335 bp 1.0 73.1