primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU_p1	trnK-UUU	1	ATCCAGAACCGGAACAAGTG	TCACATTCCACTTCGATCGT	20	20	50.0	45.0	57.8	57.0	9.5	15.9	1.215155	1498	4099	2698-2708	1.000000	71.4
trnK-UUU_p2	trnK-UUU	2	GGATCCAGAACCGGAACAAG	TCACATTCCACTTCGATCGT	20	20	55.0	45.0	58.3	57.0	9.5	15.9	1.291084	1498	4099	2700-2710	1.000000	70.8
trnK-UUU_p3	trnK-UUU	3	ATCCAGAACCGGAACAAGTG	CTCACATTCCACTTCGATCGT	20	21	50.0	47.6	57.8	58.1	9.5	15.9	1.303930	1498	4099	2699-2709	1.000000	70.7
trnK-UUU_p4	trnK-UUU	4	GGACAGAGGTACCTATCGCA	TCACATTCCACTTCGATCGT	20	20	55.0	45.0	58.3	57.0	16.3	15.9	1.341378	1498	4099	2679-2689	1.000000	70.4
trnK-UUU_p5	trnK-UUU	5	TGGACAGAGGTACCTATCGC	TCACATTCCACTTCGATCGT	20	20	55.0	45.0	58.3	57.0	16.3	15.9	1.341378	1498	4099	2680-2690	1.000000	70.4
petL-psbE_p1	petL-psbE	1	AGTAGACGTGGAAGGACTGA	TTCTCCCGTATTCCCAGACA	20	20	50.0	50.0	57.4	57.8	0.0	0.0	0.822497	13438	14702	1327-1336	1.000000	75.0
petL-psbE_p2	petL-psbE	2	AGTAGACGTGGAAGGACTGA	AATGTCAGCAAAGGAGCGTT	20	20	50.0	45.0	57.4	58.4	0.0	0.0	0.972008	13438	14702	1345-1354	1.000000	73.8
petL-psbE_p3	petL-psbE	3	GACGTGGAAGGACTGAATGA	TTCTCCCGTATTCCCAGACA	20	20	50.0	50.0	57.3	57.8	0.0	0.0	0.984916	13438	14702	1323-1332	1.000000	73.7
petL-psbE_p4	petL-psbE	4	AGACGTGGAAGGACTGAATG	TTCTCCCGTATTCCCAGACA	20	20	50.0	50.0	57.2	57.8	0.0	0.0	0.992447	13438	14702	1324-1333	1.000000	73.6
petL-psbE_p5	petL-psbE	5	AGTAGACGTGGAAGGACTGA	TCTCCCGTATTCCCAGACAT	20	20	50.0	50.0	57.4	57.5	0.0	0.0	1.053031	13438	14702	1326-1335	1.000000	73.1
cemA-ycf4_p1	cemA-ycf4	1	CACTTCCAATTCGCGAATCG	AACAGAAAGCTGCCGAATCA	20	20	50.0	45.0	57.9	58.1	32.6	0.0	0.180935	18489	18932	594-616	1.000000	81.8
cemA-ycf4_p2	cemA-ycf4	2	CACTTCCAATTCGCGAATCG	TATCGAACAGAAAGCTGCCG	20	20	50.0	50.0	57.9	58.4	32.6	0.0	0.436727	18489	18932	599-621	1.000000	79.7
cemA-ycf4_p3	cemA-ycf4	3	CTCGTCAGTTCTGTCCGAAA	AACAGAAAGCTGCCGAATCA	20	20	50.0	45.0	57.6	58.1	14.1	0.0	0.521119	18489	18932	557-579	1.000000	79.3
cemA-ycf4_p4	cemA-ycf4	4	CACTTCCAATTCGCGAATCG	AAGGGCCTACAAAACATCCC	20	20	50.0	50.0	57.9	57.8	32.6	6.4	0.308871	18489	18932	660-660	0.058824	39.9
cemA-ycf4_p5	cemA-ycf4	5	CACTTCCAATTCGCGAATCG	GGGCCTACAAAACATCCCTT	20	20	50.0	50.0	57.9	57.8	32.6	0.8	0.308871	18489	18932	658-658	0.058824	39.9
trnV-UAC-trnE-UUC_p1	trnV-UAC-trnE-UUC	1	ACGGGAAAAGCGGGTAATTT	TAAAACGCCACATGTCCGAT	20	20	45.0	45.0	58.1	57.9	0.0	19.0	0.188292	28068	28187	172-214	0.529412	68.9
trnV-UAC-trnE-UUC_p2	trnV-UAC-trnE-UUC	2	ACGGGAAAAGCGGGTAATTT	GCAAAGGGATTGAACGAACG	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.122475	28068	28187	242-284	0.529412	67.1
trnV-UAC-trnE-UUC_p3	trnV-UAC-trnE-UUC	3	ACGGGAAAAGCGGGTAATTT	GGGATTGAACGAACGGAAGA	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.175172	28068	28187	237-279	0.529412	66.9
trnV-UAC-trnE-UUC_p4	trnV-UAC-trnE-UUC	4	ACGGGAAAAGCGGGTAATTT	AGGGATTGAACGAACGGAAG	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.180984	28068	28187	238-280	0.529412	66.8
trnV-UAC-trnE-UUC_p5	trnV-UAC-trnE-UUC	5	AAGCGGGTAATTTGTCACGA	GCAAAGGGATTGAACGAACG	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.209104	28068	28187	235-277	0.529412	66.6
atpH-atpF_p1	atpH-atpF	1	TGTGGCCCTAGCACTTCTAT	CCAGCAGAAGGCCAATAACT	20	20	50.0	50.0	58.1	57.9	0.0	5.0	0.255214	47654	47996	338-436	1.000000	83.3
atpH-atpF_p2	atpH-atpF	2	ATGGACTAGTTGTGGCCCTA	CCAGCAGAAGGCCAATAACT	20	20	50.0	50.0	57.7	57.9	0.0	5.0	0.398684	47654	47996	348-446	1.000000	82.1
atpH-atpF_p3	atpH-atpF	3	TATGGACTAGTTGTGGCCCT	CCAGCAGAAGGCCAATAACT	20	20	50.0	50.0	57.7	57.9	0.0	5.0	0.398684	47654	47996	349-447	1.000000	82.1
atpH-atpF_p4	atpH-atpF	4	AGCGGAAGGTAAAATACGGG	CCAGCAGAAGGCCAATAACT	20	20	50.0	50.0	57.7	57.9	0.0	5.0	0.455534	47654	47996	413-511	1.000000	81.5
atpH-atpF_p5	atpH-atpF	5	TTGTGGCCCTAGCACTTCTA	CCAGCAGAAGGCCAATAACT	20	20	50.0	50.0	58.3	57.9	0.0	5.0	0.483052	47654	47996	339-437	1.000000	81.5
rpl14-rpl16_p1	rpl14-rpl16	1	ATTTTTCGGGCTCCACTGTT	GTCATCAGACCAGGTCGAAT	20	20	45.0	50.0	58.0	57.0	0.0	14.3	0.974252	62093	62210	275-294	1.000000	77.0
rpl14-rpl16_p2	rpl14-rpl16	2	ATTTTTCGGGCTCCACTGTT	GAAACCGTCGCTAGAGCAG	20	19	45.0	57.9	58.0	58.3	0.0	1.9	1.349685	62093	62210	230-249	1.000000	74.2
rpl14-rpl16_p3	rpl14-rpl16	3	TTTCGGGCTCCACTGTTATC	GTCATCAGACCAGGTCGAAT	20	20	50.0	50.0	57.6	57.0	0.0	14.3	1.366218	62093	62210	272-291	1.000000	73.9
rpl14-rpl16_p4	rpl14-rpl16	4	ATTTTTCGGGCTCCACTGTT	ACTTTATGAAATGGGCGGAGT	20	21	45.0	42.9	58.0	57.6	0.0	0.0	1.370676	62093	62210	255-274	1.000000	73.9
rpl14-rpl16_p5	rpl14-rpl16	5	TTTTTCGGGCTCCACTGTTA	GTCATCAGACCAGGTCGAAT	20	20	45.0	50.0	57.4	57.0	0.0	14.3	1.611782	62093	62210	274-293	1.000000	71.9
psbD-trnT-GGU_p1	psbD-trnT-GGU	1	ATAGACCAGACCAACCCACA	GGCGTAAGTCATCGGTTCAA	20	20	50.0	50.0	58.0	58.3	0.0	0.0	0.321669	82901	84214	1455-1461	1.000000	78.6
psbD-trnT-GGU_p2	psbD-trnT-GGU	2	ATAGACCAGACCAACCCACA	ATGGGGGCCTTTTCAATTCA	20	20	50.0	45.0	58.0	57.7	0.0	0.8	0.379584	82901	84214	1498-1504	1.000000	78.2
psbD-trnT-GGU_p3	psbD-trnT-GGU	3	ATAGACCAGACCAACCCACA	TGGGGGCCTTTTCAATTCAG	20	20	50.0	50.0	58.0	58.4	0.0	0.8	0.395553	82901	84214	1497-1503	1.000000	78.1
psbD-trnT-GGU_p4	psbD-trnT-GGU	4	ATAGACCAGACCAACCCACA	AATGATGGGGGCCTTTTCAA	20	20	50.0	45.0	58.0	57.7	0.0	0.8	0.379584	82901	84214	1505-1505	0.058824	39.3
psbD-trnT-GGU_p5	psbD-trnT-GGU	5	ATAGACCAGACCAACCCACA	TGATGGGGGCCTTTTCAATT	20	20	50.0	45.0	58.0	57.7	0.0	0.8	0.379584	82901	84214	1503-1503	0.058824	39.3
ycf1_p1	ycf1	1	GAAGAACGGAAGCAAACAACA	AGCTTGTACCGTTTTGTATTGA	21	22	42.9	36.4	57.6	56.3	0.0	0.0	5.116866	101000	101119	330-354	1.000000	47.2
ycf1_p2	ycf1	2	ACGGAAGCAAACAACAAATGT	AGCTTGTACCGTTTTGTATTGA	21	22	38.1	36.4	57.5	56.3	0.0	0.0	5.220951	101000	101119	325-349	1.000000	47.2
ycf1_p3	ycf1	3	TGGAACAAGGAATAGGTGCT	AGCTTGTACCGTTTTGTATTGA	20	22	45.0	36.4	56.4	56.3	0.0	0.0	5.273626	101000	101119	302-326	1.000000	47.2
ycf1_p4	ycf1	4	AAGAACGGAAGCAAACAACA	AGCTTGTACCGTTTTGTATTGA	20	22	40.0	36.4	56.4	56.3	0.0	0.0	5.306233	101000	101119	329-353	1.000000	47.2
ycf1_p5	ycf1	5	AGAACGGAAGCAAACAACAA	AGCTTGTACCGTTTTGTATTGA	20	22	40.0	36.4	56.4	56.3	0.0	0.0	5.306233	101000	101119	328-352	1.000000	47.2
rps15-psaC_p1	rps15-psaC	1	TTTGGGCAAACGTAAGCAAC	GTACGCATTGGGTACAACCA	20	20	45.0	50.0	57.8	58.2	13.3	29.0	0.400394	102555	103810	1417-1863	1.000000	77.9
rps15-psaC_p2	rps15-psaC	2	TTTGGGCAAACGTAAGCAAC	ATCTGTGGGACAAGCTCGTA	20	20	45.0	50.0	57.8	58.4	13.3	0.0	0.654254	102555	103810	1434-1880	1.000000	75.9
rps15-psaC_p3	rps15-psaC	3	GGGCAAACGTAAGCAACTTT	GTACGCATTGGGTACAACCA	20	20	45.0	50.0	57.5	58.2	13.3	29.0	0.691254	102555	103810	1414-1860	1.000000	75.6
rps15-psaC_p4	rps15-psaC	4	TGGGCAAACGTAAGCAACTT	GTACGCATTGGGTACAACCA	20	20	45.0	50.0	58.6	58.2	13.3	29.0	0.794423	102555	103810	1415-1861	1.000000	74.8
rps15-psaC_p5	rps15-psaC	5	TTGGGCAAACGTAAGCAACT	GTACGCATTGGGTACAACCA	20	20	45.0	50.0	58.6	58.2	13.3	29.0	0.794423	102555	103810	1416-1862	1.000000	74.8
rpl32-trnV-GAC_p1	rpl32-trnV-GAC	1	ATTGCGGACCGATATGCTTT	TTGACGTGGTGGAAGTCATC	20	20	45.0	50.0	58.0	57.8	20.5	0.0	0.196499	108016	108814	910-927	1.000000	83.7
rpl32-trnV-GAC_p2	rpl32-trnV-GAC	2	ATTGCGGACCGATATGCTTT	CGTGGTGGAAGTCATCAGTT	20	20	45.0	50.0	58.0	57.8	20.5	0.0	0.202514	108016	108814	906-923	1.000000	83.7
rpl32-trnV-GAC_p3	rpl32-trnV-GAC	3	ATTGCGGACCGATATGCTTT	CTTGACGTGGTGGAAGTCAT	20	20	45.0	50.0	58.0	57.8	20.5	0.0	0.202514	108016	108814	911-928	1.000000	83.7
rpl32-trnV-GAC_p4	rpl32-trnV-GAC	4	AGCTTTTATTGCGGACCGAT	TTGACGTGGTGGAAGTCATC	20	20	45.0	50.0	58.0	57.8	20.5	0.0	0.202205	108016	108814	917-934	1.000000	83.6
rpl32-trnV-GAC_p5	rpl32-trnV-GAC	5	AGCTTTTATTGCGGACCGAT	CGTGGTGGAAGTCATCAGTT	20	20	45.0	50.0	58.0	57.8	20.5	0.0	0.208219	108016	108814	913-930	1.000000	83.6
rps12-rps7_p1	rps12-rps7	1	ATGTACGGTTTGGAGGGAGA	GCAGTACTTCGTCGTGACAT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.074474	111271	111322	147-147	1.000000	81.0
rps12-rps7_p2	rps12-rps7	2	TGTACGGTTTGGAGGGAGAT	GCAGTACTTCGTCGTGACAT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.074474	111271	111322	146-146	1.000000	81.0
rps12-rps7_p3	rps12-rps7	3	ATGTACGGTTTGGAGGGAGA	TTCTGCAGTACTTCGTCGTG	20	20	50.0	50.0	58.1	58.0	0.0	14.7	0.099298	111271	111322	151-151	1.000000	80.8
rps12-rps7_p4	rps12-rps7	4	TGTACGGTTTGGAGGGAGAT	TTCTGCAGTACTTCGTCGTG	20	20	50.0	50.0	58.1	58.0	0.0	14.7	0.099298	111271	111322	150-150	1.000000	80.8
rps12-rps7_p5	rps12-rps7	5	TGTGGAAAGCCGTATTCGAC	GCAGTACTTCGTCGTGACAT	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.304264	111271	111322	176-176	1.000000	78.9
rps7-trnL-CAA_p1	rps7-trnL-CAA	1	GCAGAGGCCAATAGAGCTTT	GTCTCGCGTGTCTACCATTT	20	20	50.0	50.0	58.0	58.0	5.0	5.8	0.057719	111791	113253	1530-1573	1.000000	80.5
rps7-trnL-CAA_p2	rps7-trnL-CAA	2	GCAGAGGCCAATAGAGCTTT	CTCGCGTGTCTACCATTTCA	20	20	50.0	50.0	58.0	58.0	5.0	5.8	0.059089	111791	113253	1528-1571	1.000000	80.5
rps7-trnL-CAA_p3	rps7-trnL-CAA	3	GCAGAGGCCAATAGAGCTTT	GCGTGTCTACCATTTCACCA	20	20	50.0	50.0	58.0	58.2	5.0	0.0	0.246554	111791	113253	1525-1568	1.000000	79.0
rps7-trnL-CAA_p4	rps7-trnL-CAA	4	GCAGAGGCCAATAGAGCTTT	TCTCGCGTGTCTACCATTTC	20	20	50.0	50.0	58.0	57.7	5.0	5.8	0.323258	111791	113253	1529-1572	1.000000	78.3
rps7-trnL-CAA_p5	rps7-trnL-CAA	5	GCAGAGGCCAATAGAGCTTT	TCGCGTGTCTACCATTTCAC	20	20	50.0	50.0	58.0	58.3	5.0	5.8	0.344632	111791	113253	1527-1570	1.000000	78.2
trnL-CAA-ycf2_p1	trnL-CAA-ycf2	1	AAATGGTAGACACGCGAGAC	CAGCCAATCTCTAACCATCGA	20	21	50.0	47.6	58.0	57.5	15.9	0.0	1.467063	113335	114065	816-831	1.000000	70.2
trnL-CAA-ycf2_p2	trnL-CAA-ycf2	2	AAATGGTAGACACGCGAGAC	TCAGCCAATCTCTAACCATCG	20	21	50.0	47.6	58.0	57.5	15.9	0.0	1.467063	113335	114065	817-832	1.000000	70.2
trnL-CAA-ycf2_p3	trnL-CAA-ycf2	3	TGAAATGGTAGACACGCGAG	CAGCCAATCTCTAACCATCGA	20	21	50.0	47.6	58.0	57.5	15.9	0.0	1.468433	113335	114065	818-833	1.000000	70.2
trnL-CAA-ycf2_p4	trnL-CAA-ycf2	4	TGAAATGGTAGACACGCGAG	TCAGCCAATCTCTAACCATCG	20	21	50.0	47.6	58.0	57.5	15.9	0.0	1.468433	113335	114065	819-834	1.000000	70.2
trnL-CAA-ycf2_p5	trnL-CAA-ycf2	5	TGGTGAAATGGTAGACACGC	CAGCCAATCTCTAACCATCGA	20	21	50.0	47.6	58.2	57.5	0.0	0.0	1.655898	113335	114065	821-836	1.000000	68.7
ycf2-trnH-GUG_p1	ycf2-trnH-GUG	1	GGATTCGGTCCATTGATTGC	CCAAGGCAGTGGATTGTGAA	20	20	50.0	50.0	57.2	58.4	3.3	1.1	1.162291	120306	120629	457-577	1.000000	73.7
ycf2-trnH-GUG_p2	ycf2-trnH-GUG	2	GGATTCGGTCCATTGATTGC	TTGGTTTGTTTTTACGGGGC	20	20	50.0	45.0	57.2	57.4	3.3	0.0	1.380631	120306	120629	494-614	1.000000	71.8
ycf2-trnH-GUG_p3	ycf2-trnH-GUG	3	GGATTCGGTCCATTGATTGC	GGGTTCAATTCCCGTCGTT	20	19	50.0	52.6	57.2	58.1	3.3	0.0	1.845017	120306	120629	425-545	1.000000	68.5
ycf2-trnH-GUG_p4	ycf2-trnH-GUG	4	TCTCACCAAATTGGATTCGGT	CCAAGGCAGTGGATTGTGAA	21	20	42.9	50.0	57.6	58.4	20.3	1.1	1.813651	120306	120629	469-589	1.000000	68.4
ycf2-trnH-GUG_p5	ycf2-trnH-GUG	5	GGATTCGGTCCATTGATTGC	ACCAAGGCAGTGGATTGTG	20	19	50.0	52.6	57.2	58.0	3.3	13.6	1.822911	120306	120629	458-578	1.000000	68.4