Hotspot

ndhA

SSC · 126260-128470 · 2211 bp

Back to Abelmoschus

Pi 0.000754

MarkerSeek score 57.80

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2211
variable_sites: 3
pi: 0.000754
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAGTTACCATAGGGAACA	TGGGAGAAGTGGATCGTTGA	47.6	50.0	58.1	58.4	2336-2337 bp	1.0	68.6
ndhA_p2	TGAACCCAGTTACCATAGGGA	TGGGAGAAGTGGATCGTTGA	47.6	50.0	57.8	58.4	2339-2340 bp	1.0	67.6
ndhA_p3	TGGCCATAATTCATGAACCCA	TGGGAGAAGTGGATCGTTGA	42.9	50.0	57.6	58.4	2352-2353 bp	1.0	66.1
ndhA_p4	ACCCAGTTACCATAGGGAACA	ATGGGAGAAGTGGATCGTTG	47.6	50.0	58.1	57.3	2337-2338 bp	1.0	65.9
ndhA_p5	ACCCAGTTACCATAGGGAACA	GGGAGAAGTGGATCGTTGAA	47.6	50.0	58.1	57.2	2335-2336 bp	1.0	65.4