Hotspot

ndhF-rpl32

SSC · 118290-119258 · 969 bp

Back to Abelmoschus

Pi 0.00688

MarkerSeek score 55.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 969
variable_sites: 12
pi: 0.00688
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CCATGAATATTGATATACATGCTCCA	TGAAAAAGCTTTCAACGCCG	34.6	45.0	57.3	58.2	995-1095 bp	1.0	44.9
ndhF-rpl32_p2	CCATGAATATTGATATACATGCTCCA	GCCGCCCAATATCCTTTCTT	34.6	50.0	57.3	58.3	979-1079 bp	1.0	44.9
ndhF-rpl32_p3	TCCATGAATATTGATATACATGCTCC	TGAAAAAGCTTTCAACGCCG	34.6	45.0	57.0	58.2	996-1096 bp	1.0	44.9
ndhF-rpl32_p4	TCCATGAATATTGATATACATGCTCC	GCCGCCCAATATCCTTTCTT	34.6	50.0	57.0	58.3	980-1080 bp	1.0	44.9
ndhF-rpl32_p5	CCATGAATATTGATATACATGCTCC	TGAAAAAGCTTTCAACGCCG	36.0	45.0	55.8	58.2	995-1095 bp	1.0	44.9