Hotspot

trnN-GUU-ndhF

IRb · 114808-116087 · 1280 bp

Back to Abelmoschus

Pi 0.000781

MarkerSeek score 30.30

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 1280
variable_sites: 2
pi: 0.000781
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	CGGGAGAATCAATGACTGCA	GGGAGTCGCATCTCGTTTTA	50.0	50.0	58.0	57.7	1742-1838 bp	1.0	77.9
trnN-GUU-ndhF_p2	GGCAGAATAGCAGAGCAAGT	GGGAGTCGCATCTCGTTTTA	50.0	50.0	58.0	57.7	1839-1935 bp	1.0	77.8
trnN-GUU-ndhF_p3	AACGGTCAAAGCGAACTCTT	GGGAGTCGCATCTCGTTTTA	45.0	50.0	58.1	57.7	1516-1612 bp	1.0	77.8
trnN-GUU-ndhF_p4	ACGGTCAAAGCGAACTCTTT	GGGAGTCGCATCTCGTTTTA	45.0	50.0	58.1	57.7	1515-1611 bp	1.0	77.8
trnN-GUU-ndhF_p5	CCTCTGCGGGTAGGAAAATC	GGGAGTCGCATCTCGTTTTA	55.0	50.0	58.0	57.7	2070-2166 bp	1.0	77.7