Hotspot

petA-psbJ

LSC · 67715-68898 · 1184 bp

Back to Abelmoschus

Pi 0.000855

MarkerSeek score 61.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1184
variable_sites: 2
pi: 0.000855
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTGGC	ATTCTGGATTGGGTTCGTCC	50.0	50.0	57.8	57.6	1298-1315 bp	1.0	77.2
petA-psbJ_p2	TGAGAAGGTTCAAGTGTCCG	ATTCTGGATTGGGTTCGTCC	50.0	50.0	57.5	57.6	1230-1247 bp	1.0	74.4
petA-psbJ_p3	CGTCCAAGGCCTTTTGTTCT	ATTCTGGATTGGGTTCGTCC	50.0	50.0	58.7	57.6	1305-1322 bp	1.0	73.0
petA-psbJ_p4	GAGAAGGTTCAAGTGTCCGA	ATTCTGGATTGGGTTCGTCC	50.0	50.0	57.2	57.6	1229-1246 bp	1.0	72.1
petA-psbJ_p5	GCCTTTTGTTCTTCTTGGCA	ATTCTGGATTGGGTTCGTCC	45.0	50.0	57.1	57.6	1297-1314 bp	1.0	71.4