Hotspot

psaA-ycf3

LSC · 44622-45576 · 955 bp

Back to Abelmoschus

Pi 0.00264

MarkerSeek score 61.20

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 955
variable_sites: 5
pi: 0.00264
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	TGGTTAAAGATCACGAGGCG	50.0	50.0	57.9	58.0	1065-1079 bp	1.0	79.9
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	GGTTAAAGATCACGAGGCGT	50.0	50.0	57.9	58.0	1064-1078 bp	1.0	79.9
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	1133-1147 bp	1.0	79.6
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	1115-1129 bp	1.0	79.6
psaA-ycf3_p5	TTCTTGAGAAATGACCCGGC	TGGTTAAAGATCACGAGGCG	50.0	50.0	58.2	58.0	1088-1102 bp	1.0	79.4