Hotspot

trnT-GGU-psbD

LSC · 33811-35332 · 1522 bp

Back to Abelmoschus

Pi 0.00275

MarkerSeek score 56.80

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1522
variable_sites: 6
pi: 0.00275
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GAAACGGTCCCTACGTAACC	55.0	55.0	58.1	58.0	1647-1664 bp	1.0	80.7
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.1	58.0	1650-1667 bp	1.0	80.5
trnT-GGU-psbD_p3	ACGGCCCTTTTAACTCAGTG	GAAACGGTCCCTACGTAACC	50.0	55.0	58.1	58.0	1664-1681 bp	1.0	80.4
trnT-GGU-psbD_p4	AATTGAATCGAACGGCCCTT	GAAACGGTCCCTACGTAACC	45.0	55.0	58.2	58.0	1675-1692 bp	1.0	79.9
trnT-GGU-psbD_p5	ATTGAATCGAACGGCCCTTT	GAAACGGTCCCTACGTAACC	45.0	55.0	58.2	58.0	1674-1691 bp	1.0	79.9