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petN-psbM

LSC · 30243-31426 · 1184 bp

Back to Abelmoschus

Pi 0.00169

MarkerSeek score 61.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1184
variable_sites: 4
pi: 0.00169
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	ATTGCTACAGCGCTGTTCAT	45.0	45.0	58.0	58.3	1335-1341 bp	1.0	78.9
petN-psbM_p2	TCTTGCTTGGGCTGCTTTAA	TATTGCTACAGCGCTGTTCA	45.0	45.0	58.0	57.6	1336-1342 bp	1.0	77.9
petN-psbM_p3	AGTAAGTCTTGCTTGGGCTG	ATTGCTACAGCGCTGTTCAT	50.0	45.0	57.8	58.3	1341-1347 bp	1.0	77.4
petN-psbM_p4	TCTTGCTTGGGCTGCTTTAA	TTGCTACAGCGCTGTTCATT	45.0	45.0	58.0	58.5	1334-1340 bp	1.0	77.2
petN-psbM_p5	AGTAAGTCTTGCTTGGGCTG	TATTGCTACAGCGCTGTTCA	50.0	45.0	57.8	57.6	1342-1348 bp	1.0	76.5