Hotspot

rpoB-trnC-GCA

LSC · 28206-29500 · 1295 bp

Back to Abelmoschus

Pi 0.00722

MarkerSeek score 66.30

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1295
variable_sites: 14
pi: 0.00722
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCCATTTTCATCTCCGAGCA	CCGGATTTGAACTGGGGAAT	50.0	50.0	58.0	57.9	1354-1379 bp	1.0	81.7
rpoB-trnC-GCA_p2	CTTCTGTTAAGCCCCGATCC	CCGGATTTGAACTGGGGAAT	55.0	50.0	58.0	57.9	1433-1458 bp	1.0	81.5
rpoB-trnC-GCA_p3	CCGATCCATGAACCCACAAA	CCGGATTTGAACTGGGGAAT	50.0	50.0	58.2	57.9	1420-1445 bp	1.0	80.5
rpoB-trnC-GCA_p4	GTTAAGCCCCGATCCATGAA	CCGGATTTGAACTGGGGAAT	50.0	50.0	57.7	57.9	1428-1453 bp	1.0	79.1
rpoB-trnC-GCA_p5	TTAAGCCCCGATCCATGAAC	CCGGATTTGAACTGGGGAAT	50.0	50.0	57.7	57.9	1427-1452 bp	1.0	79.1