Hotspot

atpH-atpI

LSC · 14635-15839 · 1205 bp

Back to Abelmoschus

Pi 0.00299

MarkerSeek score 63.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1205
variable_sites: 7
pi: 0.00299
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	GCGAATCCATGGAAGGTCAT	45.0	50.0	58.0	58.0	1228-1263 bp	1.0	82.3
atpH-atpI_p2	AATAGAAGCAAGCCCGACAG	GCGAATCCATGGAAGGTCAT	50.0	50.0	58.0	58.0	1262-1297 bp	1.0	82.2
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	GCGAATCCATGGAAGGTCAT	50.0	50.0	57.9	58.0	1284-1319 bp	1.0	81.5
atpH-atpI_p4	AATAGAAGCAAGCCCGACAG	TTTTGCAACTTTAGCTGCGG	50.0	45.0	58.0	57.9	1291-1326 bp	1.0	81.3
atpH-atpI_p5	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1257-1292 bp	1.0	81.3