Hotspot

atpF-atpH

LSC · 13775-14388 · 614 bp

Back to Abelmoschus

Pi 0.00353

MarkerSeek score 64.80

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 614
variable_sites: 4
pi: 0.00353
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CAGTGACCCAAGGAAACGAA	TGGTTGTAGCATTGGCACTT	50.0	45.0	58.0	58.0	698-706 bp	1.0	82.3
atpF-atpH_p2	CAGTGACCCAAGGAAACGAA	TTGGTTGTAGCATTGGCACT	50.0	45.0	58.0	58.0	699-707 bp	1.0	82.3
atpF-atpH_p3	CAGTGACCCAAGGAAACGAA	GTTGGTTGTAGCATTGGCAC	50.0	50.0	58.0	57.9	700-708 bp	1.0	81.8
atpF-atpH_p4	CAGTGACCCAAGGAAACGAA	TGGGTTGGTTGTAGCATTGG	50.0	50.0	58.0	58.4	703-711 bp	1.0	79.3
atpF-atpH_p5	AGTGACCCAAGGAAACGAAA	TGGTTGTAGCATTGGCACTT	45.0	45.0	57.3	58.0	697-705 bp	1.0	76.8