Hotspot

ycf3

LSC · 44778-46737 · 1960 bp

Back to Abeliophyllum

Pi 0.00408

MarkerSeek score 60.10

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1960
variable_sites: 8
pi: 0.00408
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	TGACTGATGGTGCCAACAAA	TCCCACGAGAGGTAATGAGA	45.0	50.0	57.9	57.2	2103 bp	1.0	73.2
ycf3_p2	TTGACTGATGGTGCCAACAA	TCCCACGAGAGGTAATGAGA	45.0	50.0	57.9	57.2	2104 bp	1.0	73.2
ycf3_p3	TTTGACTGATGGTGCCAACA	TCCCACGAGAGGTAATGAGA	45.0	50.0	57.9	57.2	2105 bp	1.0	73.2
ycf3_p4	ACTGATGGTGCCAACAAACA	TCCCACGAGAGGTAATGAGA	45.0	50.0	58.2	57.2	2101 bp	1.0	71.9
ycf3_p5	TGACTGATGGTGCCAACAAA	TCCCACGAGAGGTAATGAGAA	45.0	47.6	57.9	57.9	2103 bp	1.0	71.0