Hotspot

rps19

LSC · 86466-86744 · 279 bp

Back to Abeliophyllum

Pi 0

MarkerSeek score 27.40

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: LSC
length_bp: 279
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 27.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	TGTCGACCTAAAGCGTATACT	TTGATTCTTCGTCGCCGTAG	42.9	50.0	56.3	58.1	464 bp	1.0	57.7
rps19_p2	TGTCGACCTAAAGCGTATACT	ATTTGATTCTTCGTCGCCGT	42.9	45.0	56.3	58.3	466 bp	1.0	56.3
rps19_p3	TGTCGACCTAAAGCGTATACT	TTTGATTCTTCGTCGCCGTA	42.9	45.0	56.3	57.6	465 bp	1.0	55.7
rps19_p4	TGTCGACCTAAAGCGTATACTT	TTGATTCTTCGTCGCCGTAG	40.9	50.0	57.0	58.1	464 bp	1.0	55.5
rps19_p5	CGACCTAAAGCGTATACTTCTGT	TTGATTCTTCGTCGCCGTAG	43.5	50.0	58.1	58.1	461 bp	1.0	54.6