Pi
0.00134
MarkerSeek score
59.50
Primer available
yes
Label
rpl2
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| rpl2_p1 | GCCACAGCTTCCTCCTATTT | CGCTTCAACCGGGTTATTCT | 50.0 | 50.0 | 57.6 | 58.3 | 1620 bp | 1.0 | 74.6 |
| rpl2_p2 | GCCACAGCTTCCTCCTATTT | ATCATTACGCTTCAACCGGG | 50.0 | 50.0 | 57.6 | 58.3 | 1627 bp | 1.0 | 74.0 |
| rpl2_p3 | TGCCACAGCTTCCTCCTATT | CGCTTCAACCGGGTTATTCT | 50.0 | 50.0 | 58.7 | 58.3 | 1621 bp | 1.0 | 72.3 |
| rpl2_p4 | GCCACAGCTTCCTCCTATTT | ACGCTTCAACCGGGTTATTC | 50.0 | 50.0 | 57.6 | 58.6 | 1621 bp | 1.0 | 72.3 |
| rpl2_p5 | TGCCACAGCTTCCTCCTATT | ATCATTACGCTTCAACCGGG | 50.0 | 50.0 | 58.7 | 58.3 | 1628 bp | 1.0 | 71.7 |
| rpl2_p1 | CGCTTCAACCGGGTTATTCT | ACAGCTTCCTCCTATTTTGT | 50.0 | 40.0 | 58.3 | 54.2 | 1617 bp | 1.0 | 47.8 |
| rpl2_p2 | ATCATTACGCTTCAACCGGG | ACAGCTTCCTCCTATTTTGT | 50.0 | 40.0 | 58.3 | 54.2 | 1624 bp | 1.0 | 47.3 |
| rpl2_p3 | CGCTTCAACCGGGTTATTCT | ACAGCTTCCTCCTATTTTGTT | 50.0 | 38.1 | 58.3 | 55.1 | 1617 bp | 1.0 | 46.8 |
| rpl2_p4 | ATCATTACGCTTCAACCGGG | ACAGCTTCCTCCTATTTTGTT | 50.0 | 38.1 | 58.3 | 55.1 | 1624 bp | 1.0 | 46.2 |
| rpl2_p5 | ACGCTTCAACCGGGTTATTC | ACAGCTTCCTCCTATTTTGT | 50.0 | 40.0 | 58.6 | 54.2 | 1618 bp | 1.0 | 45.5 |