Hotspot

petD

LSC · 79241-80436 · 1196 bp

Back to Abeliophyllum

Pi 0

MarkerSeek score 31.90

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1196
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	GGAGTTGAAGGGAATTCTCCG	TCCCTAGATACACACGTCGT	52.4	50.0	58.4	57.6	1279 bp	1.0	65.8
petD_p2	GAGTTGAAGGGAATTCTCCGA	TCCCTAGATACACACGTCGT	47.6	50.0	57.4	57.6	1278 bp	1.0	63.8
petD_p3	TGGAGTTGAAGGGAATTCTCC	TCCCTAGATACACACGTCGT	47.6	50.0	57.3	57.6	1280 bp	1.0	63.0
petD_p4	AGTTGAAGGGAATTCTCCGA	TCCCTAGATACACACGTCGT	45.0	50.0	56.2	57.6	1277 bp	1.0	62.0
petD_p5	TGAAGGGAATTCTCCGAAGAG	TCCCTAGATACACACGTCGT	47.6	50.0	57.1	57.6	1274 bp	1.0	61.6