Hotspot

trnK-UUU-rps16

LSC · 4408-5251 · 844 bp

Back to Abeliophyllum

Pi 0.00118

MarkerSeek score 59.40

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 844
variable_sites: 1
pi: 0.00118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CGAATTCCTCGCTTCATCCA	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.1	58.2	1016 bp	1.0	78.1
trnK-UUU-rps16_p2	AAAGCCGAGTACTCTACCGT	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.2	58.2	968 bp	1.0	77.2
trnK-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.2	58.2	967 bp	1.0	77.2
trnK-UUU-rps16_p4	CCGCACTTAAAAGCCGAGTA	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.3	58.2	977 bp	1.0	76.3
trnK-UUU-rps16_p5	TACCGTTGAGTTAGCAACCC	GGTGCTCAACCTACAGGAAC	50.0	55.0	57.5	58.2	954 bp	1.0	74.9