Hotspot

matK-trnK-UUU

LSC · 3660-4370 · 711 bp

Back to Abeliophyllum

Pi 0.00141

MarkerSeek score 59.60

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 711
variable_sites: 1
pi: 0.00141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	CCTGATCATGAGCAAGTGCA	AACGGTAGAGTACTCGGCTT	50.0	50.0	58.3	58.2	841 bp	1.0	76.7
matK-trnK-UUU_p2	CCTGATCATGAGCAAGTGCA	ACGGTAGAGTACTCGGCTTT	50.0	50.0	58.3	58.2	840 bp	1.0	76.7
matK-trnK-UUU_p3	ACCCTGATCATGAGCAAGTG	AACGGTAGAGTACTCGGCTT	50.0	50.0	57.6	58.2	843 bp	1.0	75.4
matK-trnK-UUU_p4	ACCCTGATCATGAGCAAGTG	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.6	58.2	842 bp	1.0	75.4
matK-trnK-UUU_p5	CCTGATCATGAGCAAGTGCA	GGGTTGCTAACTCAACGGTA	50.0	50.0	58.3	57.5	854 bp	1.0	74.4