primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	matK-trnK-UUU	1	CCTGATCATGAGCAAGTGCA	AACGGTAGAGTACTCGGCTT	20	20	50.0	50.0	58.3	58.2	15.6	17.1	0.421950	3660	4370	841-841	1.000000	76.7
matK-trnK-UUU_p2	matK-trnK-UUU	2	CCTGATCATGAGCAAGTGCA	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	58.3	58.2	15.6	17.1	0.421950	3660	4370	840-840	1.000000	76.7
matK-trnK-UUU_p3	matK-trnK-UUU	3	ACCCTGATCATGAGCAAGTG	AACGGTAGAGTACTCGGCTT	20	20	50.0	50.0	57.6	58.2	15.6	17.1	0.583413	3660	4370	843-843	1.000000	75.4
matK-trnK-UUU_p4	matK-trnK-UUU	4	ACCCTGATCATGAGCAAGTG	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	57.6	58.2	15.6	17.1	0.583413	3660	4370	842-842	1.000000	75.4
matK-trnK-UUU_p5	matK-trnK-UUU	5	CCTGATCATGAGCAAGTGCA	GGGTTGCTAACTCAACGGTA	20	20	50.0	50.0	58.3	57.5	15.6	0.0	0.714287	3660	4370	854-854	1.000000	74.4
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CGAATTCCTCGCTTCATCCA	GGTGCTCAACCTACAGGAAC	20	20	50.0	55.0	58.1	58.2	0.0	1.3	0.250342	4408	5251	1016-1016	1.000000	78.1
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAAGCCGAGTACTCTACCGT	GGTGCTCAACCTACAGGAAC	20	20	50.0	55.0	58.2	58.2	20.3	1.3	0.358736	4408	5251	968-968	1.000000	77.2
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAGCCGAGTACTCTACCGTT	GGTGCTCAACCTACAGGAAC	20	20	50.0	55.0	58.2	58.2	20.3	1.3	0.358736	4408	5251	967-967	1.000000	77.2
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CCGCACTTAAAAGCCGAGTA	GGTGCTCAACCTACAGGAAC	20	20	50.0	55.0	58.3	58.2	5.3	1.3	0.473122	4408	5251	977-977	1.000000	76.3
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TACCGTTGAGTTAGCAACCC	GGTGCTCAACCTACAGGAAC	20	20	50.0	55.0	57.5	58.2	0.0	1.3	0.651073	4408	5251	954-954	1.000000	74.9
rpoC2_p1	rpoC2	1	TACGACACGGATCGGGATTA	CCATTTTCCTGGAGAGACCG	20	20	50.0	55.0	58.0	58.0	14.1	0.0	0.063904	18862	19011	950-950	0.500000	59.5
rpoC2_p2	rpoC2	2	TACGACACGGATCGGGATTA	GGGTTCAGGATTGCTCTCTG	20	20	50.0	55.0	58.0	58.0	14.1	0.0	0.069595	18862	19011	458-458	0.500000	59.4
rpoC2_p3	rpoC2	3	TACGACACGGATCGGGATTA	CCACCAGGAACAGGAAAACA	20	20	50.0	50.0	58.0	57.9	14.1	0.0	0.099964	18862	19011	898-898	0.500000	59.2
rpoC2_p4	rpoC2	4	TACGACACGGATCGGGATTA	ACCACCAGGAACAGGAAAAC	20	20	50.0	50.0	58.0	57.9	14.1	0.0	0.101431	18862	19011	899-899	0.500000	59.2
rpoC2_p5	rpoC2	5	AATGTTACGACACGGATCGG	CCATTTTCCTGGAGAGACCG	20	20	50.0	55.0	58.1	58.0	14.1	0.0	0.112566	18862	19011	955-955	0.500000	59.1
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	CTCATTTCCATCCCCGAGC	CCGGATTTGAACTGGGGAAA	19	20	57.9	50.0	58.0	58.1	0.0	0.0	1.094688	28023	29217	1278-1278	1.000000	71.3
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	ATTCCCTCATTTCCATCCCC	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	56.9	58.1	0.0	0.0	1.163184	28023	29217	1283-1283	1.000000	70.8
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	TCATTTCCATCCCCGAGCAT	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	59.2	58.1	0.0	0.0	1.236113	28023	29217	1277-1277	1.000000	70.2
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	CTCATTTCCATCCCCGAGC	AAAAGGATTTGCAGTCCCCC	19	20	57.9	50.0	58.0	58.4	0.0	14.6	1.368051	28023	29217	1260-1260	1.000000	69.1
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	ACATTCCCTCATTTCCATCCC	CCGGATTTGAACTGGGGAAA	21	20	47.6	50.0	57.7	58.1	0.0	0.0	1.401159	28023	29217	1285-1285	1.000000	68.9
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AGTAGAAAAGCGGTAGGAACT	TTCAATTGGTCAGAGCACCG	21	20	42.9	50.0	56.4	58.5	0.0	5.4	3.088455	31457	32502	1178-1199	1.000000	55.6
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AGCGGTAGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	20	20	45.0	50.0	55.4	58.5	0.0	5.4	3.107053	31457	32502	1170-1191	1.000000	55.4
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AAGCGGTAGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	21	20	42.9	50.0	56.2	58.5	0.0	5.4	3.298996	31457	32502	1171-1192	1.000000	53.9
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AGCGGTAGGAACTAGAATGAA	TTCAATTGGTCAGAGCACCG	21	20	42.9	50.0	56.2	58.5	0.0	5.4	3.298996	31457	32502	1170-1191	1.000000	53.9
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AAGTAGAAAAGCGGTAGGAACT	TTCAATTGGTCAGAGCACCG	22	20	40.9	50.0	57.1	58.5	0.0	5.4	3.363827	31457	32502	1179-1200	1.000000	53.4
ycf3_p1	ycf3	1	TGACTGATGGTGCCAACAAA	TCCCACGAGAGGTAATGAGA	20	20	45.0	50.0	57.9	57.2	2.9	0.0	0.885161	44778	46737	2103-2103	1.000000	73.2
ycf3_p2	ycf3	2	TTGACTGATGGTGCCAACAA	TCCCACGAGAGGTAATGAGA	20	20	45.0	50.0	57.9	57.2	2.9	0.0	0.885161	44778	46737	2104-2104	1.000000	73.2
ycf3_p3	ycf3	3	TTTGACTGATGGTGCCAACA	TCCCACGAGAGGTAATGAGA	20	20	45.0	50.0	57.9	57.2	1.3	0.0	0.885161	44778	46737	2105-2105	1.000000	73.2
ycf3_p4	ycf3	4	ACTGATGGTGCCAACAAACA	TCCCACGAGAGGTAATGAGA	20	20	45.0	50.0	58.2	57.2	2.9	0.0	1.046618	44778	46737	2101-2101	1.000000	71.9
ycf3_p5	ycf3	5	TGACTGATGGTGCCAACAAA	TCCCACGAGAGGTAATGAGAA	20	21	45.0	47.6	57.9	57.9	2.9	0.0	1.160345	44778	46737	2103-2103	1.000000	71.0
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	48975	49711	787-787	1.000000	79.0
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	48975	49711	795-795	1.000000	78.5
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	TAAGGAAAAGCCGGCTATCG	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	33.1	0.0	0.238923	48975	49711	838-838	1.000000	78.2
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	48975	49711	819-819	1.000000	78.1
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	TAAGGAAAAGCCGGCTATCG	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	33.1	0.0	0.303732	48975	49711	846-846	1.000000	77.7
trnV-UAC_p1	trnV-UAC	1	ATTTCGATGAACCAGCCCAA	ACCACTCAAAAATGGAGGATGT	20	22	45.0	40.9	57.8	57.9	0.0	0.0	2.332545	54117	54764	839-839	1.000000	61.5
trnV-UAC_p2	trnV-UAC	2	GATTTCGATGAACCAGCCCA	ACCACTCAAAAATGGAGGATGT	20	22	50.0	40.9	58.2	57.9	0.0	0.0	2.367976	54117	54764	840-840	1.000000	61.2
trnV-UAC_p3	trnV-UAC	3	TGATTTCGATGAACCAGCCC	ACCACTCAAAAATGGAGGATGT	20	22	50.0	40.9	58.2	57.9	0.0	0.0	2.367976	54117	54764	841-841	1.000000	61.2
trnV-UAC_p4	trnV-UAC	4	AACCGGTCAGTTACAAGCAA	ACCACTCAAAAATGGAGGATGT	20	22	45.0	40.9	57.7	57.9	8.0	0.0	2.453484	54117	54764	821-821	1.000000	60.6
trnV-UAC_p5	trnV-UAC	5	ACCGGTCAGTTACAAGCAAC	ACCACTCAAAAATGGAGGATGT	20	22	50.0	40.9	58.4	57.9	0.0	0.0	2.528366	54117	54764	820-820	1.000000	60.0
rbcL_p1	rbcL	1	AACGCCTAATTCGTGTCGAG	AAGATTGGGCCGAGTTTTCA	20	20	50.0	45.0	58.4	57.7	0.0	0.0	0.660749	57903	59336	1590-1590	1.000000	74.9
rbcL_p2	rbcL	2	AACGCCTAATTCGTGTCGAG	AGATTGGGCCGAGTTTTCAA	20	20	50.0	45.0	58.4	57.7	0.0	0.0	0.660749	57903	59336	1589-1589	1.000000	74.9
rbcL_p3	rbcL	3	CGCCTAATTCGTGTCGAGTA	AAGATTGGGCCGAGTTTTCA	20	20	50.0	45.0	57.5	57.7	0.0	0.0	0.749917	57903	59336	1588-1588	1.000000	74.2
rbcL_p4	rbcL	4	CGCCTAATTCGTGTCGAGTA	AGATTGGGCCGAGTTTTCAA	20	20	50.0	45.0	57.5	57.7	0.0	0.0	0.749917	57903	59336	1587-1587	1.000000	74.2
rbcL_p5	rbcL	5	TAACGCCTAATTCGTGTCGA	AGATTGGGCCGAGTTTTCAA	20	20	45.0	45.0	57.1	57.7	0.0	0.0	1.204065	57903	59336	1590-1590	1.000000	70.5
rpl20-rps12_p1	rpl20-rps12	1	TCCGAGCTATATATCCGCGT	AAATGTCACGAAATCCCCCG	20	20	50.0	50.0	58.0	58.5	4.9	0.0	0.574654	71699	72490	893-893	1.000000	75.5
rpl20-rps12_p2	rpl20-rps12	2	CTTGAATGAGCCCCTCGAAA	AAATGTCACGAAATCCCCCG	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.654410	71699	72490	948-948	1.000000	74.8
rpl20-rps12_p3	rpl20-rps12	3	TTGAATGAGCCCCTCGAAAG	AAATGTCACGAAATCCCCCG	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.654410	71699	72490	947-947	1.000000	74.8
rpl20-rps12_p4	rpl20-rps12	4	TCCGAGCTATATATCCGCGT	CAGCGTCGAGGAACATGTAC	20	20	50.0	55.0	58.0	58.7	4.9	9.8	0.764938	71699	72490	856-856	1.000000	74.0
rpl20-rps12_p5	rpl20-rps12	5	TTGAATGAGCCCCTCGAAAG	CAGCGTCGAGGAACATGTAC	20	20	50.0	55.0	57.9	58.7	0.0	9.8	0.844694	71699	72490	910-910	1.000000	73.3
petD_p1	petD	1	GGAGTTGAAGGGAATTCTCCG	TCCCTAGATACACACGTCGT	21	20	52.4	50.0	58.4	57.6	0.4	0.0	1.774783	79241	80436	1279-1279	1.000000	65.8
petD_p2	petD	2	GAGTTGAAGGGAATTCTCCGA	TCCCTAGATACACACGTCGT	21	20	47.6	50.0	57.4	57.6	0.4	0.0	2.020037	79241	80436	1278-1278	1.000000	63.8
petD_p3	petD	3	TGGAGTTGAAGGGAATTCTCC	TCCCTAGATACACACGTCGT	21	20	47.6	50.0	57.3	57.6	0.4	0.0	2.129358	79241	80436	1280-1280	1.000000	63.0
petD_p4	petD	4	AGTTGAAGGGAATTCTCCGA	TCCCTAGATACACACGTCGT	20	20	45.0	50.0	56.2	57.6	0.4	0.0	2.251508	79241	80436	1277-1277	1.000000	62.0
petD_p5	petD	5	TGAAGGGAATTCTCCGAAGAG	TCCCTAGATACACACGTCGT	21	20	47.6	50.0	57.1	57.6	0.4	0.0	2.303503	79241	80436	1274-1274	1.000000	61.6
rps19_p1	rps19	1	TGTCGACCTAAAGCGTATACT	TTGATTCTTCGTCGCCGTAG	21	20	42.9	50.0	56.3	58.1	14.1	0.0	2.783661	86466	86744	464-464	1.000000	57.7
rps19_p2	rps19	2	TGTCGACCTAAAGCGTATACT	ATTTGATTCTTCGTCGCCGT	21	20	42.9	45.0	56.3	58.3	14.1	0.0	2.965794	86466	86744	466-466	1.000000	56.3
rps19_p3	rps19	3	TGTCGACCTAAAGCGTATACT	TTTGATTCTTCGTCGCCGTA	21	20	42.9	45.0	56.3	57.6	14.1	0.0	3.038704	86466	86744	465-465	1.000000	55.7
rps19_p4	rps19	4	TGTCGACCTAAAGCGTATACTT	TTGATTCTTCGTCGCCGTAG	22	20	40.9	50.0	57.0	58.1	14.1	0.0	3.066518	86466	86744	464-464	1.000000	55.5
rps19_p5	rps19	5	CGACCTAAAGCGTATACTTCTGT	TTGATTCTTCGTCGCCGTAG	23	20	43.5	50.0	58.1	58.1	0.6	0.0	3.177819	86466	86744	461-461	1.000000	54.6
rpl2_p1	rpl2	1	GCCACAGCTTCCTCCTATTT	CGCTTCAACCGGGTTATTCT	20	20	50.0	50.0	57.6	58.3	0.0	13.0	0.692680	86800	88291	1620-1620	1.000000	74.6
rpl2_p2	rpl2	2	GCCACAGCTTCCTCCTATTT	ATCATTACGCTTCAACCGGG	20	20	50.0	50.0	57.6	58.3	0.0	6.3	0.763148	86800	88291	1627-1627	1.000000	74.0
rpl2_p3	rpl2	3	TGCCACAGCTTCCTCCTATT	CGCTTCAACCGGGTTATTCT	20	20	50.0	50.0	58.7	58.3	0.0	13.0	0.976677	86800	88291	1621-1621	1.000000	72.3
rpl2_p4	rpl2	4	GCCACAGCTTCCTCCTATTT	ACGCTTCAACCGGGTTATTC	20	20	50.0	50.0	57.6	58.6	0.0	13.0	0.980972	86800	88291	1621-1621	1.000000	72.3
rpl2_p5	rpl2	5	TGCCACAGCTTCCTCCTATT	ATCATTACGCTTCAACCGGG	20	20	50.0	50.0	58.7	58.3	0.0	6.3	1.047145	86800	88291	1628-1628	1.000000	71.7
trnA-UGC_p1	trnA-UGC	1	TGATTTACTTCACGGGCGAG	GGTGCTGCTAAATGGATGGA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.061720	105632	106517	1116-1117	1.000000	79.6
trnA-UGC_p2	trnA-UGC	2	TGATTTACTTCACGGGCGAG	AGGTGCTGCTAAATGGATGG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.067553	105632	106517	1117-1118	1.000000	79.6
trnA-UGC_p3	trnA-UGC	3	TGATTTACTTCACGGGCGAG	AATCATAGATCGAACCGCCG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.070575	105632	106517	1072-1073	1.000000	79.6
trnA-UGC_p4	trnA-UGC	4	TGATTTACTTCACGGGCGAG	CATAGATCGAACCGCCGAAT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.070575	105632	106517	1069-1070	1.000000	79.6
trnA-UGC_p5	trnA-UGC	5	GCGCCAGGGAAAAGAATAGA	GGTGCTGCTAAATGGATGGA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.095524	105632	106517	1065-1066	1.000000	79.4
ndhH_p1	ndhH	1	AGGGACAAGCAACCATATGA	TCATGGAAGAATGACTCGGT	20	20	45.0	45.0	56.5	56.3	3.7	0.0	3.243699	123811	124992	1311-1311	1.000000	54.1
ndhH_p2	ndhH	2	ACAAGCAACCATATGATCCCA	TCATGGAAGAATGACTCGGT	21	20	42.9	45.0	57.3	56.3	3.7	0.0	3.413253	123811	124992	1307-1307	1.000000	52.8
ndhH_p3	ndhH	3	AGGGACAAGCAACCATATGA	TTCATGGAAGAATGACTCGGT	20	21	45.0	42.9	56.5	57.0	3.7	0.0	3.479658	123811	124992	1312-1312	1.000000	52.2
ndhH_p4	ndhH	4	ACAAGCAACCATATGATCCCA	TTCATGGAAGAATGACTCGGT	21	21	42.9	42.9	57.3	57.0	3.7	0.0	3.649212	123811	124992	1308-1308	1.000000	50.9
ndhH_p5	ndhH	5	AGGGACAAGCAACCATATGAT	TCATGGAAGAATGACTCGGT	21	20	42.9	45.0	57.0	56.3	3.7	0.0	3.704607	123811	124992	1311-1311	1.000000	50.4
trnA-UGC_p1	trnA-UGC	1	GGTGCTGCTAAATGGATGGA	TGATTTACTTCACGGGCGAG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.061720	136208	137093	1116-1117	1.000000	79.6
trnA-UGC_p2	trnA-UGC	2	AGGTGCTGCTAAATGGATGG	TGATTTACTTCACGGGCGAG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.067553	136208	137093	1117-1118	1.000000	79.6
trnA-UGC_p3	trnA-UGC	3	AATCATAGATCGAACCGCCG	TGATTTACTTCACGGGCGAG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.070575	136208	137093	1072-1073	1.000000	79.6
trnA-UGC_p4	trnA-UGC	4	CATAGATCGAACCGCCGAAT	TGATTTACTTCACGGGCGAG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.070575	136208	137093	1069-1070	1.000000	79.6
trnA-UGC_p5	trnA-UGC	5	GGTGCTGCTAAATGGATGGA	GCGCCAGGGAAAAGAATAGA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.095524	136208	137093	1065-1066	1.000000	79.4
rpl2_p1	rpl2	1	CGCTTCAACCGGGTTATTCT	ACAGCTTCCTCCTATTTTGT	20	20	50.0	40.0	58.3	54.2	13.0	0.0	4.035282	154434	155925	1617-1617	1.000000	47.8
rpl2_p2	rpl2	2	ATCATTACGCTTCAACCGGG	ACAGCTTCCTCCTATTTTGT	20	20	50.0	40.0	58.3	54.2	6.3	0.0	4.105750	154434	155925	1624-1624	1.000000	47.3
rpl2_p3	rpl2	3	CGCTTCAACCGGGTTATTCT	ACAGCTTCCTCCTATTTTGTT	20	21	50.0	38.1	58.3	55.1	13.0	0.0	4.166463	154434	155925	1617-1617	1.000000	46.8
rpl2_p4	rpl2	4	ATCATTACGCTTCAACCGGG	ACAGCTTCCTCCTATTTTGTT	20	21	50.0	38.1	58.3	55.1	6.3	0.0	4.236931	154434	155925	1624-1624	1.000000	46.2
rpl2_p5	rpl2	5	ACGCTTCAACCGGGTTATTC	ACAGCTTCCTCCTATTTTGT	20	20	50.0	40.0	58.6	54.2	13.0	0.0	4.323574	154434	155925	1618-1618	1.000000	45.5