Hotspot

ycf2

IRa · 149690-156614 · 6925 bp

Back to Abelia

Pi 0.00834
MarkerSeek score 46.60
Primer available yes
Label ycf2

Feature Metadata

label_name
ycf2
feature_type
gene
region
IRa
length_bp
6925
variable_sites
149
pi
0.00834
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf2_p1 GGGAAGTATCATGATCGTCCA TCCGATTGATTGGTCTGAAGT 47.6 42.9 57.0 57.0 332-341 bp 1.0 59.9
ycf2_p2 TGGGAAGTATCATGATCGTCC TCCGATTGATTGGTCTGAAGT 47.6 42.9 57.0 57.0 333-342 bp 1.0 59.9
ycf2_p3 GGGAAGTATCATGATCGTCCA TCCGATTGATTGGTCTGAAGTT 47.6 40.9 57.0 57.7 332-341 bp 1.0 57.5
ycf2_p4 TGGGAAGTATCATGATCGTCC TCCGATTGATTGGTCTGAAGTT 47.6 40.9 57.0 57.7 333-342 bp 1.0 57.5
ycf2_p5 GGGAAGTATCATGATCGTCCAA TCCGATTGATTGGTCTGAAGT 45.5 42.9 57.7 57.0 332-341 bp 1.0 57.5