Hotspot

trnL-CAA-ycf2

IRa · 148768-149689 · 922 bp

Back to Abelia

Pi 0.0126
MarkerSeek score 58.60
Primer available yes
Label trnL-CAA-ycf2

Feature Metadata

label_name
trnL-CAA-ycf2
feature_type
igs
region
IRa
length_bp
922
variable_sites
42
pi
0.0126
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-CAA-ycf2_p1 AAATGGTAGACACGCGAGAC ACAGTATGCGCTTCAAGAGG 50.0 50.0 58.0 58.0 1435-1446 bp 0.8 76.4
trnL-CAA-ycf2_p2 TGAAATGGTAGACACGCGAG ACAGTATGCGCTTCAAGAGG 50.0 50.0 58.0 58.0 1437-1448 bp 0.8 76.4
trnL-CAA-ycf2_p3 CCAACACTCCACCTTTGTCA ACAGTATGCGCTTCAAGAGG 50.0 50.0 57.9 58.0 1529-1540 bp 0.8 75.8
trnL-CAA-ycf2_p4 AAATGGTAGACACGCGAGAC CAAGAGGGTGGTGGAGAGTA 50.0 55.0 58.0 58.1 1422-1431 bp 0.6 67.7
trnL-CAA-ycf2_p5 TGAAATGGTAGACACGCGAG CAAGAGGGTGGTGGAGAGTA 50.0 55.0 58.0 58.1 1424-1433 bp 0.6 67.7